AIM:To identify the fungi by lacto phenol cotton blue staining
MATERIALS REQUIRED:
Slide
Inoculation loop
Bunsen burner
Fungal Culture
Cover slip
LPCB stain
PROCEDURE:
Take a clean dry glass slide
A drop of LBCB stain was placed in the centre of the slide
With the help of inoculation loop a small portion of colony was scratched and placed in the drop of stain
Then it was teased into small bits with the help of teasing needle
A cover slip was placed on top and gentle pressure was applied for even spreading
It was then observed under the microscope
MATERIALS REQUIRED:
Slide
Inoculation loop
Bunsen burner
Fungal Culture
Cover slip
LPCB stain
PROCEDURE:
Take a clean dry glass slide
A drop of LBCB stain was placed in the centre of the slide
With the help of inoculation loop a small portion of colony was scratched and placed in the drop of stain
Then it was teased into small bits with the help of teasing needle
A cover slip was placed on top and gentle pressure was applied for even spreading
It was then observed under the microscope
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